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Research papers

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  1. Andrew E. Maclean**,  Megan A. Sloan, Eléa A. Renaud,  Vincent Demolombe,  Sébastien Besteiro,  Lilach Sheiner**; The Toxoplasma gondii mitochondrial transporter ABCB7 is essential for cytosolic iron-sulfur cluster biogenesis and protein translation. doi: https://doi.org/10.1101/2024.03.15.585200

  2. Ovciarikova J, Shikha S, Lacombe A, Courjol F, McCrone R, Hussain W, Maclean A, Lemgruber L, Martins-Duarte ES, Gissot M, Sheiner L.** Two ancient membrane pores mediate mitochondrial-nucleus membrane contact sites.  J Cell Biol. 2024 Apr 1;223(4):e202304075. doi: 10.1083/jcb.202304075. Epub 2024 Mar 8. PMID: 38456969

  3. Pradeep Sheokand,  Alexander Mühleip,  Lilach Sheiner** Plasmodium falciparum mitochondrial complex III, the target of atovaquone, is essential for progression to the transmissible sexual stages. BioRxiv.  doi: https://doi.org/10.1101/2024.01.09.574740

  4. Silva MF, Douglas K, Sandalli S, Maclean AE**, Sheiner L**. Functional and biochemical characterization of the Toxoplasma gondii succinate dehydrogenase complex. PLoS Pathog. 2023 Dec 11;19(12):e1011867. doi: 10.1371/journal.ppat.1011867. eCollection 2023 Dec. PMID: 38079448 

  5. Elati HAA, Goerner AL, Martorelli Di Genova B, Sheiner L, de Koning HP. Pyrimidine salvage in Toxoplasma gondii as a target for new treatment. Front Cell Infect Microbiol. 2023 Dec 15;13:1320160. doi: 10.3389/fcimb.2023.1320160. eCollection 2023.

  6. Shikha Shikha, Mariana Ferreira Silva, and Lilach Sheiner** Identification and Validation of Toxoplasma gondii Mitoribosomal Large Subunit Components. Microorganisms 2022, 10(5), 863; https://doi.org/10.3390/microorganisms10050863

  7. Gustavo D. Campagnaro, Hamza A. A. Elati, Sofia Balaska, Maria Esther Martin Abril, Manal J. Natto, Fabian Hulpia, Kelly Lee, Lilach Sheiner, Serge Van Calenbergh, Harry P. De Koning. A Toxoplasma gondii oxopurine transporter binds nucleobases and nucleosides in different orientations. Int. J. Mol. Sci. 2022.

  8. Jitka Richtová, Lilach Sheiner, Ansgar Gruber,Shun-Min Yang, Luděk Kořený, Boris Striepen and Miroslav Oborník. Using Diatom and Apicomplexan Models to Study the Heme Pathway of Chromera velia. Int. J. Mol. Sci. 2021, 22(12), 6495; https://doi.org/10.3390/ijms22126495 

  9. Érica S Martins-Duarte, Lilach Sheiner, Sarah B Reiff, Wanderley de Souza, Boris Striepen. Replication and partitioning of the apicoplast genome of Toxoplasma gondii is linked to the cell cycle and requires DNA polymerase and gyrase. Int J Parasitol. 2021 Feb 10S0020-7519(21)00047-3. doi: 10.1016/j.ijpara.2020.11.004. 

  10. Mühleip A, Flygaard RK, Ovciarikova J, Lacombe A, Fernandes P, Sheiner L,** Amunts A.** ATP synthase hexamer assemblies shape cristae of Toxoplasma mitochondria. Nat Commu  2021 Jan 5;12(1):120. doi: 10.1038/s41467-020-20381-z.

  11. Mallo N, Martins Duarte ES, Baehr SC, Biddau M, Ovciarikova J, Wilde ML,  Uboldi AD, Lemgruber L, Tonkin JC, Wideman JG, Harding CR**, Sheiner L** Depletion of voltage-dependent anion channel (VDAC) of Toxoplasma gondii affects multiple mitochondrial functions, but not calcium signalling J Cell Sci. 2021 Sep 15:jcs.255299. doi: 10.1242/jcs.255299. Online ahead of print.PMID: 34523684

  12. Maclean AE.** Bridges HR.  Silva MF. Ding S. Hirst J.  Sheiner L.** Complexome profile of Toxoplasma gondii mitochondria identifies a divergent cytochrome bc1 complex  doi: https://doi.org/10.1101/2020.08.17.252163

  13. Biddau M**. Kumar S. Henrich P. Laine LM. Blackburn GJ. Chokkathukalam A. Li TB. Sim LK. Hoffman SL.  Barrett MP. Coombs GH. McFadden GI. Fidock DA*. Müller S*. Sheiner L.** Lipoic acid biosynthesis is essential for Plasmodium falciparum transmission and influences redox response and carbon metabolism of parasite asexual blood stages doi: https://doi.org/10.1101/2020.05.17.099630

  14. Rodríguez LJ. Mallo N. Biddau M. Fernandes F. de Miguel T. Sheiner L. Choupina A. Lores M. Exploring the powerful phytoarsenal of white grape marc against bacteria and parasites causing significant diseases Environ Sci Pollut Res Int. 2020 Jan 14. doi: 10.1007/s11356-019-07472-1.

  15. Melatti C, Pieperhoff M, Lemgruber L, Pohl E, Sheiner L**, and Meissner M**. A unique dynamin-related protein is essential for mitochondrial fission in Toxoplasma gondii. PLoS Pathog. 2019 Apr 4;15(4):e1007512. doi: 10.1371/journal.ppat.1007512

  16. Lacombe A, Maclean AE, Ovciarikova J, Tottey J and Sheiner L**. In silico screen identifies a new Toxoplasma gondii mitochondrial ribosomal protein essential for mitochondrial translation. Mol Microbiol. 2019 Oct;112(4):1235-1252. doi: 10.1111/mmi.14357

  17. Shahar Bracha**, Karoliina Hassi, Paul D. Ross, Stuart Cobb, Lilach Sheiner* **  and Oded Rechavi* **. Engineering Brain Parasites for Intracellular Delivery of Therapeutic Proteins. bioRxiv 481192; doi: https://doi.org/10.1101/481192 Nat Microbiol . 2024 Jul 29. doi: 10.1038/s41564-024-01750-6.

  18. Alison Mbekeani, Will Stanley, Vishal Kalel, Noa Dahan, Einat Zalckvar, Lilach Sheiner, Wolfgang Schliebs, Ralf Erdmann, Ehmke Pohl and Paul Denny. Functional analyses of a putative, membrane-bound, peroxisomal protein import mechanism from the apicomplexan protozoan Toxoplasma gondii. Gene, Special Issue "Membrane Proteins in Parasitic Protozoa". Genes 2018, 9(9), 434; https://doi.org/10.3390/genes9090434 

  19. Biddau M*, Bouchut A*, Major J*, Saveria T*, Tottey J*, Oka O, van-Lith M, Jennings KE, Ovciarikova J, DeRocher A, Striepen B, Waller RF, Parsons M and Sheiner L** Two essential Thioredoxins mediate apicoplast biogenesis, protein import, and gene expression in Toxoplasma gondii. PLoS ppathogens. 2018 Feb 22;14(2):e1006836

  20. Jana Ovciarikova, Leandro Lemgruber, Krista L. Stilge, William J. Sulliva & Lilach Sheiner** Mitochondrial behaviour throughout the lytic cycle of Toxoplasma gondii Scientific Reports 2017, Feb 16;7:42746. doi: 10.1038/srep42746

  21. Sheiner L**, Fellows JD, Ovciarikova J, Brooks CF, Agrawal S, Holmes ZC, Bietz I, Flinner N, Heiny S, Mirus O, Przyborski JM, Striepen B. Toxoplasma gondii Toc75 functions in import of stromal but not peripheral apicoplast proteins. Traffic. 2015 Sep 18. doi: 10.1111/tra.12333.

  22. Williams MJ, Alonso H, Enciso M, Egarter S, Sheiner L, Meissner M, Striepen B, Smith BJ, Tonkin CJ. Two Essential Light Chains Regulate the MyoA Lever Arm To Promote Toxoplasma Gliding Motility.MBio. 2015 Sep 15;6(5). pii: e00845-15. doi: 10.1128/mBio.00845-15.

  23. Daher W, Morlon-Guyot J, Sheiner L, Lentini G, Berry L, Tawk L, Dubremetz JF, Wengelnik K, Striepen B, Lebrun M. Lipid kinases are essential for apicoplast homeostasis in Toxoplasma gondii. Cell Microbiol. 2014 Oct 20. doi: 10.1111/cmi.12383.

  24. Francia F, Jordan C, Patel J, Sheiner L, Demerly JD, Brooks CF, Fellow J, de Leon JC, Dubremetz JF, Morrissette NS, Striepen B. Cell division in apicomplexan parasites is organized by a homolog of the striated rootlet fiber of algal flagella. PLoS Biol. 2012 Dec;10(12):e1001444

  25. MacRae JI*, Sheiner L*, Nahid A, Tonkin C, Striepen B and McConville MJ. Mitochondrial metabolism of glucose and glutamine is required for intracellular growth of Toxoplasma gondii. Cell Host Microbe. 2012 Nov 15;12(5):682-92

  26. Sampels V, Hartmann A, Dietrich I, Coppens I, Sheiner L, Striepen B, Herrmann A, Lucius R, Gupta N. Conditional mutagenesis of a novel choline kinase demonstrates plasticity of phosphatidylcholine biogenesis and gene expression in Toxoplasma gondii. J Biol Chem. 2012 May 11;287(20):16289-99. doi: 10.1074/jbc.M112.347138

  27. Sheiner L**, Demerly JL, Poulsen N, Beatty W, Lucas O, Behnke MS, White MW, and Striepen B.  A Systematic Screen to Discover and Analyze Apicoplast Proteins Identifies a Conserved and Essential Protein Import Factor. PLoS Pathogens. 2011 Dec;7(12):e1002392.

  28. Lorestani A, Sheiner L, Yang K, Robertson SD, Sahoo N, Brooks CF, Ferguson DJ, Striepen B, and Marc-Jan Gubbels. (2010) A Toxoplasma MORN1 null mutant undergoes repeated divisions but is defective in basal assembly, apicoplast division and cytokinesis. PLoS One 19;5(8):e12302.

  29. Sheiner L*, Santos JM*, Klages N, Parussini F, Jemmely N, Friedrich N, Ward GE, Soldati-Favre D. Toxoplasma gondii transmembrane microneme proteins and their modular design. Mol Microbiol. 2010 Jun 9. doi:  10.1111/j.1365-2958.2010.07255.x

  30. Pino P, Aeby E*, Foth BJ*, Sheiner L*, Soldati T, Schneider A, Soldati-Favre D. Mitochondrial translation in absence of local tRNA aminoacylation and methionyl tRNA Met formylation in Apicomplexa. Mol Microbiol. 2010 May;76(3):706-18. doi: 10.1111/j.1365-2958.2010.07128.x.

  31. Goldshmidt H, Sheiner L, Butikofer P, Roditi I, Uliel S, Günzel M, Engstler M and Michaeli S. (2008) Role of protein translocation pathways across the endoplasmic reticulum in Trypanosoma brucei. J Biol Chem. 14;283(46):32085-98.

  32. Sheiner L, Dowse TJ, and Soldati D. (2008) Identification of trafficking determinants for polytopic rhomboid proteases in Toxoplasma gondii. Traffic. 2008 May;9(5):665-77. doi: 10.1111/j.1600-0854.2008.00736.x

  33. Lustig Y, Sheiner L, Vagima Y, Goldshmidt H, Das A, Bellofatto V and Michaeli S. Spliced-leader RNA silencing: a novel stress-induced mechanism in Trypanosoma brucei. EMBO Rep. 2007 Apr;8(4):408-13.

  34. Pino P, Foth B, Kwok LY, Sheiner L, Schepers R, Soldati T, and Soldati-Favre D. Dual targeting of antioxidant and metabolic enzymes to the mitochondrion and the apicoplast of Toxoplasma gondii. PLoS Pathog. 2007 Aug 31;3(8):e115.

*Authors with equal contribution.

**Coresponding author

 

 

Review papers

 

  1. The mystery of massive mitochondrial complexes: the apicomplexan respiratory chain. Maclean AE, Hayward JA, Huet D, van Dooren GG, Sheiner L** Trends Parasitol. 2022 Dec;38(12):1041-1052. doi: 10.1016/j.pt.2022.09.008. Epub 2022 Oct 24.

  2. Toxoplasma metabolic flexibility in different growth conditions.Walsh D, Katris NJ, Sheiner L**, Botté CY**. Trends Parasitol. 2022 Jun 16:S1471-4922(22)00132-5. doi: 10.1016/j.pt.2022.06.001. PMID: 35718642

  3. Protein control of membrane and organelle dynamics: Insights from the divergent eukaryote Toxoplasma gondii. JanaOvciarikova, Rodolpho Ornitz Oliveira Souza, Gustavo Arrizabalaga, Lilach Sheiner**Current Opinion in Cell Biology. Volume 76, June 2022, 102085. https://doi.org/10.1016/j.ceb.2022.102085

  4. Nuclear Interactions: A Spotlight on Nuclear Mitochondrial Membrane Contact Sites. Jana Ovciarikova*, Shikha Shikha*, Lilach Sheiner** Contact 2022. https://doi.org/10.1177/25152564221096217

  5. Biddau M**, Sheiner L**.  Targeting the apicoplast in malaria. Biochem Soc Trans. 2019 Aug 30;47(4):973-983. doi: 10.1042/BST20170563. 

  6. Natalia Mallo*, Justin Fellows*, Carla Johnson and Lilach Sheiner**. Protein Import into the Endosymbiotic Organelles of Apicomplexan Parasites. Genes 2018, 9(8), 412; https://doi.org/10.3390/genes9080412 

  7. Sheiner L**, Vaidya AB, and McFadden GI. The metabolic roles of the endosymbiotic organelles of Toxoplasma and Plasmodium spp. Curr Opin Microbiol. 2013 Aug;16(4):452-8. doi: 10.1016/j.mib.2013.07.003

  8. Sheiner L, Striepen B. Protein sorting in complex plastids. Biochim Biophys Acta. 2013 Feb;1833(2):352-9. doi: 10.1016/

  9. Sheiner L and Soldati-Favre D. Protein trafficking inside Toxoplasma gondii. Traffic. 2008 May;9(5):636-46. doi: 10.1111/j.1600-0854.2008.00713.x

 

Book chapters

 

  1. Jacot D, Lourido S., Meissner M, Sheiner L, Soldati-Favre D and Striepen B. (2020) Genetic Manipulation of T. gondii. Toxoplasma Gondii (Third Edition). Chapter 20; Pages 897-940. (Editors:Kim k, and Weiss L).

  2. Biddau M, Ovciarikova J, Sheiner L. Experimental Approaches for Examining Apicoplast Biology. Methods Mol Biol. 2020;2071:221-243. doi: 10.1007/978-1-4939-9857-9_13.

  3. Sheiner L and Striepen B. (2014) The Apicoplast: A Parasite's Symbiont. Plastid Biology, Advance in Plan Biology 5, DOI 10.1007/978-1-4939-1136-3_8, Springer.

  4. Jacot D, Meissner M, Sheiner L, Soldati-Favre D and Striepen B. (2014) Genetic Manipulation of T. gondii. Toxoplasma Gondii (Second Edition). Chapter 17; Pages 577–611. (Editors:Kim k, and Weiss L).

  5. Agrawal S, Nair S, Sheiner L, Striepen B. The Apicoplast: An ancient algal endosymbiont of Apicomplexa (2011) Book chapter, STRUCTURES AND ORGANELLES IN PATHOGENIC PROTISTS (Editor: Wanderley de Souza) MICROBIOLOGY MONOGRAPHS (Springer)

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About MeBOP

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Sheiner L**, Harb OS**. MeBoP: Advanced Training in the Biology of Parasitism for Middle Eastern Students.   

Trends Parasitol. 2019 May;35(5):329-332. doi: 10.1016/j.pt.2019.03.001.

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